Talk:Structural Biochemistry/Proteins/Gel Electrophoresis

2-Dimensional Gel Electrophoresis
2-Dimensional Gel Electrophoresis is a method used to separate proteins by size and isoelectric point. The samples are loaded into the wells on an agarose gel submerged in a salt-buffer. A current is run through the aparatus and the proteins travel through the porous gel. Small proteins travel further as they fit through the pores easier than larger proteins. What's left is bands of proteins separated by size. Once this is completed and the proteins are spread out enough to identify their sizes, the gel is rotated 90 degrees and the current is applied again. These two categories allow the proteins to be separated by not only mass/size, but isoelectric point, which will differentiate them further from each other. (Under construction...)