Proteomics/Protein Separations- Electrophoresis/Timelines of Electrophoresis


 * 1) Gel Electrophoresis
 * 2) One Dimensional Gel Electrophoresis
 * 3) Two Dimensional Polyacrylamide Gel Electrophoresis(2D-PAGE)
 * 4) Differential in Gel Electrophoresis(DIGE)
 * 5) QPNC-PAGE
 * 6) Capillary Electrophoresis
 * 7) Timeline of Electrophoresis
 * 8) Databases
 * 9) Web Pages
 * 10) Online Applications
 * 11) Further Readings

Timeline of Electrophoresis
1930s:  Use of sucrose for gel electrophoresis.

1937:  The official launching of Tiselius' moving boundary electrophoresis (MBE).

1939:  Zone Electrophoresis

1950:  Rich in introducing novel methodologies in zone electrophoresis (ZE)

1950:  Agar Gel electrophoresis

1955:  Introduction of starch gels

1957:  Cellulose acetate Electrophoresis (CAc) by Kohn

1959:  Introduction of acrylamide gels by Raymond and Winstraub

1961:  Electrophoresis in silica gel by Honnegar

1961: IsoElectricFocusing by Svensson

1964:  Electrophoresis in thin layer of sephadex beads by Fasella et.al

1964:  Disc gel electrophoresis Ornstein and Davis

1969:  Introduction of denaturing agents especially SDS separation of protein subunit by Beber and Osborn

1970:  T4 phage proteins were separated using stacking gel and SDS by Laemmli

1975:  2 Dimensional gels: Isoelectric focusing and SDS gel electrophoresis by OFarrell.

1977:  Sequencing gels

1970s:  agars gels

1980s: Immobilized pH gradients

1983:  Pulsed field gel electrophoresis

1983:  Capillary electrophoresis