Proteomics/Protein Separations- Electrophoresis/Timeline of Electrophoresis


 * 1) Introduction to Electrophoresis
 * 2) Gel Electrophoresis
 * 3) One Dimentional Gel Electrophoresis
 * 4) Two Dimensional Polyacrylamide Gel Electrophoresis(2D-PAGE)
 * 5) Differential in Gel Electrophoresis(DIGE)
 * 6) QPNC-PAGE
 * 7) Capillary Electrophoresis
 * 8) Timeline of Electrophoresis
 * 9) Databases
 * 10) Web Pages
 * 11) Online Applications
 * 12) Further Readings

Timeline of Electrophoresis
1930s :  Use of sucrose for gel electrophoresis.

1937 :  The official launching of Tiselius' moving boundary electrophoresis (MBE).

1939 :  Zone Electrophoresis

1950 :  Rich in introducing novel methodologies in zone electrophoresis (ZE)

1950 :  Agar Gel electrophoresis

1955 :  Introduction of starch gels

1957 :  Cellulose acetate Electrophoresis(CAc) by Kohn

1959 :  Arrival of Acrylamide gels (Raymond and Winstraub)

1961 :  Electrophoresis in silica gel by Honnegar

1961 : IsoElectricFocusing by Svensson

1964 :  Electrophoresis in thin layer of sephadex beads by Fasella et.al

1964 :  Ornstein and Davis introduced Disc-Gel Electrophoresis.

1969 :  Beber and Osborn introduced denaturing agents i.e. SDS separation of protein subunit

1970 :  Stacking gel and SDS by Laemmli was used to separate T4 phage proteins

1975 :  2-D gels :Isoelectric focussing and SDS gel electrophoresis by OFarrell.

1977 :  Arrival of Sequencing gels

1970s :  Arrival of Agarose gels

1980s : Immobilized pH gradients

1983 :  Pulsed field gel electrophoresis was invented

1983 :  Introduction of Capillary electrophoresis

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Subcription Based References

 * Righetti PG, Electrophoresis: the march of pennies, the march of dimes; Journal of Chromatography A. 2005 Jun 24;1079(1-2):24-40. Abstract[]