Proteomics/Protein Separations- Electrophoresis/Further Readings

Open Source Articles

 * Discussion of the strengths and weakness of using 2-D gel electrophoresis coupled with mass spectrometry for proteomics research
 * Beranova-Giorgianni, S. (2003)  Proteome analysis by two-dimensional gel electrophoresis and mass spectrometry:  strengths and limitations.   Trends in Analytical Chemistry 22(5), 273-281.[]


 * Discuss the capabilities of 2DGE when combined with mass spectrometry in proteome analysis
 * Stephen J Fey and Peter Mose Larsen(2001) 2D or not 2D: Current Opinion in Chemical Biology 2001, 5:26–33[]


 * Discusses a variant of QPNC-PAGE
 * Kastenholz, B. 2004. Preparative native continuous polyacrylamide gel electrophoresis (PNC-PAGE): An efficient method for isolating cadmium cofactors in biological systems. Anal. Lett. 37, 657-665.


 * Contributions of QPNC-PAGE
 * Kastenholz, B. 2006. Important contributions of a new quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE) procedure for elucidating metal cofactor metabolisms in protein-misfolding diseases – a theory. Protein Pept. Lett. 13, 503-508. Special issue [Hot Topic: Recent Advances in Protein and Peptide Chemistry Related to Cellular Regulation (Guest Editor: John W. Ho)].

Subscription Required Articles

 * Review of high-throughput separation methods including a discussion on gel-electrophoresis based techniques
 * Suresh, S., Mohan, S., Mishra, G., Hanumanthu, G., Suresh, M., Reddy, R., Pandey, A. (2005)  Proteomic resources:  Integrating biomedical information in humans.  Gene 364, 13-18.  Abstact[]


 * Discuss the merits of 2D-PAGE when coupled with ICAT in quantitative proteome analysis
 * Marcus Smolka‡, Huilin Zhou§, and Ruedi Aebersold§(2002) Quantitative Protein Profiling Using Two-dimensional Gel electrophoresis,Isotope-coded Affinity Tag Labeling, and Mass Spectrometry. Molecular & Cellular Proteomics 1:19–29,[]


 * Discuss the merits and drawbacks of 2DGE and ICAT
 * Wayne F Patton*, Birte Schulenberg and Thomas H Steinberg(2002) Two-dimensional gel electrophoresis; better than a poke in the ICAT?. Current Opinion in Biotechnology, 13:321–328 Abstract []


 * Highlights the advances of 2D gels in quantitative proteomics
 * Kathryn S Lilley, Azam Razzaq and Paul Dupree(2001)Two-dimensional gel electrophoresis: recent advances in sample preparation, detection and quantitation. Current Opinion in Chemical Biology, 6:46–50 Abstact[]


 * Review of historical and current multi-dimensional peptide separation methods
 * Issaq, H., Chan, K., Janini, G., Conrads, T., Veenstra, T. (2005)  Multidimensional separation of peptides for effective proteomic analysis.  Journal of Chromatography B 817 (1), 35-47.

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