GENtle/Calculators

The calculator module can be invoked via Tools/Calculator. It contains several specialized spreadsheet-based calculators for typical tasks in molecular miology. The editable fields are shown in blue, the (major) results of the calculation are shown in red.

Ligation calculator
This calculator gives the amount (in µl) of vector and insert for a ligation, based on the length and concentration of each respectively, their desired ratio and total mass of DNA. A typical ratio of insert:vector is 3:1

DNA concentration calculator
This calculator gives the amount and purity of DNA based on photometric absorption at 260 and 280 nm, respectively, as well as the dilution (in case one measures a 1:100 dilution of the original DNA sample) and a correction factor for different types of nucleic acids.

Protein calculator
This calculator gives the amount and purity of peptides/proteins based on photometric absorption at 250 and 280 nm, respectively, as well as the molecular weight of the peptide, the layer thickness of the cuvette used, and the number of tryptophanes, tyrosines and cysteines in the peptide.

Data
This shows a codon table and a reverse codon table, both for standard code. This page can not be edited.