Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Fluorogenic

Fluorogenic refers to a process in which fluorescence is generated. The term is generally used in the context of analytical assays, where a chemical reaction dependent on the presence of a particular analyte produces fluorescent molecules. By using a spectrophotometer, the amount of fluorescence (and indirectly, the amount of analyte) can be quantitated. Fluorogenic methods are typically several orders of magnitude more sensitive than the older chromogenic methods. Chromogenic is the term for the generation of color, rather than fluorescence.